ASCP (American Society for Clinical Pathology) Phlebotomy Technician Certification Practice Exam

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What is a possible occurrence if a blood specimen tube for hematology tests is filled correctly but not mixed long enough?

Hemolysis of the specimen

Tiny clots will form in the specimen

When a blood specimen tube for hematology tests is filled correctly but not mixed thoroughly, tiny clots are likely to form in the specimen. Mixing is essential after filling the tube to ensure that the anticoagulant (if used) is evenly distributed throughout the blood sample. If the sample isn't mixed properly, the anticoagulant may not work effectively, leading to the coagulation of blood cells, which can result in the formation of small clots. These clots can interfere with various hematological tests, causing inaccurate results. This situation emphasizes the importance of proper mixing to ensure the integrity of the sample and reliable test outcomes.

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Inaccurate test results due to dilution

Contamination with air bubbles

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